Reduced CCR5 expression in Ethiopian commercial sex workers with CCR2b-64l polymorphism
| Id | EPHI-DS0123 |
|---|---|
| Name | Reduced CCR5 expression in Ethiopian commercial sex workers with CCR2b-64l polymorphism |
| Format | .dta |
| Coverage Location | National |
| Coverage Sex | Female |
| Abstract | A cross-sectional study was conducted on HIV-1-infected and non-infected Ethiopian commercial sex workers (CSW). The aim of the study was to determine expression levels of CCR5 and CXCR4 and to study the association of CCR5 expression with CCR2b polymorphism (CCR2b-64l). The study determines CCR5 and CXCR4 expression in 64 female Ethiopian commercial sex workers (32 HIV" and 32 HIV+) and CCR5 expression in relation to CCR2b genotype in 40 of these women (23 HIV" and 17 HIV+) after informed consent to participate in the study. The expression pattern of the HIV-1 co-receptors in the two study groups determined. In that regard, the expression of CCR5 was found to be Upregulated in CD45RA+CD4+ and total CD4+ T cells of HIV-- infected subjects compared to the uninfected group, whereas the expression level of CXCR4 in CD45RO+CD4+ and total CD4+ T cells was reduced in the HIV-1-positive CSW. The overall frequency of the CCR2b-64l polymorphism was 55% in the CSW. There was no significant difference in the percentage of the CCR2b-64l genotype between the HIV-1-infected (64.7%) and -non-infected (47.8%) subjects. Remarkably, within the HIV-1-negative CSW, a significantly reduced CCR5 expression on total CD4+ T cells was observed in individuals with CCR2b-64l genotype compared to CCR2b wild type. This association was not present in HIV-1-infected CSW. Despite reduced CCR5 expression in vivo, in-vitro susceptibility and p24 production after infection with different HIV-1 biological isolates were not different between HIV-1-negative CSW with CCR2b wild-type and mutant genotype. The results of this study suggest that CCR2b- 64I might mediate its disease progression delaying effect by affecting CCR5 expression. |
| Additional Material | No |
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| Recommended | Yes |
| Location | |
| Cleaned | Yes |
| Cleaned Format | . csdb |
| RawFormat | . csdb |
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| Date Data Collection Started | 1999-05-20 |
| Date Data Collection End | 1999-08-30 |
| Title | Reduced CCR5 expression in Ethiopian commercial sex workers with CCR2b-64l polymorphism |
| Data Type | Survey |
| PublicationYear | 2001 |
| SugestedCitation | no suggested citation |
| OtherIdType | |
| Description | The study is a cross-sectional regional representative study conducted among 104 HIV-infected and non-infected commercial sex workers in Addis Ababa. Expression of CCR5 was found to be upregulated in CD45RA+CD4+ and total CD4+ T cells of HIV- infected subjects compared to the uninfected group, whereas the expression level of CXCR4 in CD45 RO+CD4+ and total CD4+ T cells was reduced in the HIV-1-positive CSW. The overall frequency of the CCR2b-64l polymorphism was 55% in the CSW. There was no significant difference in the percentage of the CCR2b-64l genotype between the HIV-1-infected (64.7%) and -non-infected (47.8%) subjects. Remarkably, within the HIV-1-negative CSW, a significantly reduced CCR5 expression on total CD4+ T cells was observed in individuals with CCR2b-64ll genotype compared to CCR2b wild type. This association was not present in HIV-1-infected CSW. Despite reduced CCR5 expression in-vitro susceptibility and p24 production after infection with different HIV-1 biological isolates were not different between HIV-1-negative CSW with CCR2b wild-type and mutant genotype. The results of this study suggest that CCR2b- 64I might mediate its disease progression delaying effect by affecting CCR5 expression. |
| Dataset study design | Clinical trials |
| Date Data Archived | 2020-08-15 |
| Date Data Cataloged | 2020-09-08 |
| Data Generating Unit | HIV and TB Research Directorate |
| URL | https://rtds.ephi.gov.et/public/showdetail/123 |
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Open Access