The difference in in-vitro susceptibility to HIV-1 between high-risk HIV-negative Ethiopian commercial sex workers and low-risk controls
| Id | EPHI-DS0122 |
|---|---|
| Name | The difference in in-vitro susceptibility to HIV-1 between high-risk HIV-negative Ethiopian commercial sex workers and low-risk controls |
| Format | .dta |
| Coverage Location | Regional |
| Coverage Sex | Female |
| Abstract | The study aimed to compare the in-vitro susceptibility of the high-risk commercial sex workers of Addis Ababa who work for 5 years and low-risk controls. Hostt factors such as increased p-chemokine production, HIV-1 coreceptor expression level, and HIV-1 co-receptor polymorphism have been thought to influence susceptibility to HIV-1 infection. To determine the protective role of these factors in Ethiopians who remained HIV-1- uninfected, despite multiple high-risk sexual exposures, 21 Ethiopian women were studied, who had been employed as commercial sex workers (CSW) for five or more years. The HIV-1- resistant CSW were compared to low-risk age-matched female controls who had a comparable CD4 percentage and mean fluorescence intensity (MFl). Genetic polymorphism in the CCR5, CCR2b or SDF-11 genes appeared to be not associated with resistance in the Ethiopian CSW, Expression levels of CCR5 and CXCR4 on naïve, memory and total CD4*T cells tended to be higher in the resistant CSW, while the production of fc-chemokines RANTES, MIP1-a, and MIP1-fi by phytohemagglutinin (PHA)- stimulated peripheral blood mononuclear cells (PBMC) was lower compared to low-risk HIV-1-negative controls. In-vitro susceptibility of PHA-stimulated PBMCC to primary, CCR5-restricted, Ethiopian HIV-1 isolates was comparable between resistant CSW and low-risk controls. In-vitro susceptibility was positively correlated to CD4 mean fluorescence intensity and negatively correlated to CCR5 expression levels, suggesting that infection of PBMC was primarily dependent on expression levels of CD4 and that CCR5 expression, above a certain threshold, did not further increase susceptibility. The results show that co-receptor polymorphism, co-receptor expression levels, fi-chemokine production, and cellular resistance to in-vitro HIV-1 infection are not associated with protection in high-risk HIV-1-negative Ethiopian CSW. |
| Additional Material | No |
| Keywords |
|
| Recommended | Yes |
| Location | |
| Cleaned | Yes |
| Cleaned Format | . csdb |
| RawFormat | . csdb |
| Comment | |
| Remark | |
| Note | |
| Treatment | |
| Date Data Collection Started | 1998-05-25 |
| Date Data Collection End | 1998-08-29 |
| Title | The difference in in-vitro susceptibility to HIV-1 between high-riskk HIV-negative Ethiopian commercial sex workers and low-risk controls |
| Data Type | Survey |
| PublicationYear | 2004 |
| SugestedCitation | no sugested citation |
| OtherIdType | |
| Description | The study is a regional representative cross-sectional study among 372 High risk and commercial sex workers and low-risk controls in Addis Ababa. The study aimed to compare the in vitro susceptibility of the low-risk and high-risk commercial sex workers. All study subjects gave their informed consent to participate in the study Ethical clearance for the study was obtained from both EHNRI and National ethical Clearance Committees. In this study, the high-risk HIV-1 negative group consisted of 21 female CSW living in Addis Ababa who reported to have been working as CSW for five or more years. HIV-11 testing was performed using a combination of a rapid assay (HIVSPOT, Gene-labs Diagnostics, Singapore) and an ELISA (Vironostika HIV Uniform plus O, Organon Teknika, Boxtel, The Netherlands). In that regard, statistical analyses were performed using the STATA program (Stata Corporation, Texas, USA). Biological factors and susceptibility were compared between two groups using non-parametric methods (Mann-Whitney U test). Spearman correlation coefficients (r) were used to describe correlations between continuous variables. Finally, mutation frequencies for HIV-1 co-receptors were compared between two groups by Fisher exact test. |
| Dataset study design | Longitudinal |
| Date Data Archived | 2020-08-15 |
| Date Data Cataloged | 2020-04-09 |
| Data Generating Unit | HIV and TB Research Directorate |
| URL | https://rtds.ephi.gov.et/public/showdetail/122 |
| Tags |
|
Open Access